產(chǎn)品詳情介紹
ProteinIso? Ni-NTA Resin是螯合金屬Ni2+而形成的一種親和層析介質。NTA能夠通過四個位點牢固的螯合Ni2+，從而減少純化過程中Ni2+泄露到蛋白樣品中。本產(chǎn)品對His標簽蛋白具有特異吸附能力，從而使His標簽蛋白結合在Ni-NTA純化介質上，未結合的蛋白被洗滌下去，結合在介質上的蛋白經(jīng)過一定濃度的咪唑或低pH緩沖液溫和洗脫下來，從而得到高純度的目的蛋白。本產(chǎn)品具有吸附容量大、選擇性好，通透性強，易于再生的優(yōu)點。它可用于在非變性或變性條件下純化任何表達系統(tǒng)表達的His標簽重組蛋白。
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2 Liu Y, Yu T F, Li Y T, et al. Mitogen‐activated protein kinase TaMPK3 suppresses ABA response by destabilising TaPYL4 receptor in wheat[J]. New Phytologist, 2022.(IF 10.32)
3 Guo L, Zhao Y, Zhang Q, et al. Stochastically multimerized ParB orchestrates DNA assembly as unveiled by single-molecule analysis[J]. Nucleic Acids Research, 2022.(IF 19.16)
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5 Zhang Q, Chen Z, Wang F, et al. Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer[J]. Nucleic Acids Research, 2021.(IF 16.97)
