產(chǎn)品詳情介紹
本產(chǎn)品以甲基化的質(zhì)粒為模板，采用部分重疊引物 (均含突變點(diǎn)) 設(shè)計(jì)，使用2×TransStart? FastPfu Fly PCR SuperMix擴(kuò)增，擴(kuò)增產(chǎn)物用DMT限制性內(nèi)切酶消化甲基化質(zhì)粒模板后，轉(zhuǎn)化具有降解甲基化質(zhì)粒模板的感受態(tài)細(xì)胞。本產(chǎn)品具有突變效率高，操作簡便的優(yōu)點(diǎn)。
1 Yang F, Chen Y, Liu Q, et al. Dynamics and regulations of BimEL Ser65 and Thr112 phosphorylation in porcine granulosa cells during follicular atresia[J]. Cells, 2020.(IF 5.27)
2 Ke Y, Lv X, Fu X, et al. Poly (ADP-ribosyl) ation enhances HuR oligomerization and contributes to pro-inflammatory gene mRNA stabilization[J]. Cellular and Molecular Life Sciences, 2021.(IF 6.49)
3 Ouyang M, Li X, Zhang J, et al. Liquid-liquid phase transition drives intra-chloroplast cargo sorting[J]. Cell, 2020.(IF 66.85)
4 Song N, Qi Q, Cao R, et al. MAVS O-GlcNAcylation is essential for host antiviral immunity against lethal RNA viruses[J]. Cell reports, 2019.(IF 7.81)
5 Hu Z, He J, Chen K, et al. Molecular cloning and biochemical characterization of a new flavonoid glycosyltransferase from the aquatic plant lotus[J]. Biochemical and biophysical research communications, 2019.(IF 12.10)
6 You L, Shi J, Shen L, et al. Structural basis for transcription antitermination at bacterial intrinsic terminator[J]. Nature communications, 2019.(IF 11.80)
7 Liu M, Xu B, Ma Y, et al. Reversible covalent inhibitors suppress enterovirus 71 infection by targeting the 3C protease[J]. Antiviral Research, 2021.(IF 5.97)
8 Liu X, Liu Z, Wu Z, et al. Resurrection of endogenous retroviruses during aging reinforces senescence[J]. Cell, 2023.(IF 66.85)
9 Tian Y, Chen Z H, Wu P, et al. MIR497HG‐Derived miR‐195 and miR‐497 Mediate Tamoxifen Resistance via PI3K/AKT Signaling in Breast Cancer[J]. Advanced Science, 2023.(IF 17.52)
10 Wang K, Zhang Z, Hang J, et al. Microbial-host-isozyme analyses reveal microbial DPP4 as a potential antidiabetic target[J]. Science, 2023.(IF 56.90)
11 Shi C, Yang X, Hou Y, et al. USP15 promotes cGAS activation through deubiquitylation and liquid condensation[J]. Nucleic Acids Research, 2022.(IF 14.90)
12 Chen Y G, Li D S, Ling Y, et al. A cryptic plant terpene cyclase producing unconventional 18‐and 14‐membered macrocyclic C25 and C20 terpenoids with immunosuppressive activity[J]. Angewandte Chemie, 2021.(IF 15.33)
13 Huang K, Wu X X, Fang C L, et al. Pol IV and RDR2: a two-RNA-polymerase machine that produces double-stranded RNA[J]. Science, 2021.(IF 63.71)
