pEASY?-T5 Zero Cloning Kit
T5基因克隆試劑盒(雙抗性、零背景、無(wú)藍(lán)白斑篩選)
目錄號(hào): CT501-01
單 價(jià):¥860
產(chǎn)品詳情介紹
該載體通過(guò)自殺基因表達(dá)與否篩選陽(yáng)性重組子。當(dāng)載體與片段連接成功時(shí),自殺基因無(wú)法正確表達(dá),包含重組子的細(xì)胞可以正常生長(zhǎng);當(dāng)載體與片段連接不成功時(shí),自殺基因正確表達(dá),包含載體的細(xì)胞無(wú)法生長(zhǎng),即“Zero”背景。適用于TA克隆。
1 Chen W, Chen L, Zhang X, et al. Convergent selection of a WD40 protein that enhances grain yield in maize and rice[J]. Science, 2022.(IF 63.71)
2 Zhao Z, Zhang Z, Li J, et al. Sustained TNF-α stimulation leads to transcriptional memory that greatly enhances signal sensitivity and robustness[J]. Elife, 2020.(IF 7.08)