產品詳情介紹
TransStart? FastPfu DNA Polymerase 是用于快速PCR的熱啟動高保真DNA聚合酶，TransStart? FastPfu DNA Polymerase擴增效率高、擴增速度快(4 kb/min，是普通Pfu酶的8倍)，克服了普通Pfu酶擴增效率低、產量低和擴增速度慢(0.5 kb/min)的缺陷，極大地縮短了反應時間。
· 保真性是EasyTaq? DNA Polymerase的54倍。
· 擴增產物為平端，可直接克隆于pEASY?-Blunt系列載體中。
· 基因組DNA片段的擴增 (≤ 15 kb)。
· Plasmid DNA片段的擴增 (≤ 20 kb)。
1 Fan J, Ran H, Wei P L, et al. Pretrichodermamide A Biosynthesis Reveals the Hidden Diversity of Epidithiodiketopiperazines[J]. Angewandte Chemie, 2023.(IF 16.82)
2 Wang Y, Zhang S, Yang X, et al. Mesoscale DNA feature in antibody-coding sequence facilitates somatic hypermutation[J]. Cell, 2023.(IF 66.00)
3 Fan J, Ran H, Wei P L, et al. An ortho‐Quinone Methide Mediates Disulfide Migration in the Biosynthesis of Epidithiodiketopiperazines[J]. Angewandte Chemie International Edition, 2023.(IF 16.82)
4 Zong Y, Liu Y, Xue C, et al. An engineered prime editor with enhanced editing efficiency in plants[J]. Nature Biotechnology, 2022.(IF 54)
5 Lei Y, Fei P, Song B, et al. A loosened gating mechanism of RIG-I leads to autoimmune disorders[J]. Nucleic acids research, 2022.(IF 16.971)
6 Zhang H, Zhu Y, Liu Z, et al. A volatile from the skin microbiota of flavivirus-infected hosts promotes mosquito attractiveness[J]. Cell, 2022.(IF 66.85)
7 Zhang H, Li Z, Zhou S, et al. A fungal NRPS-PKS enzyme catalyses the formation of the flavonoid naringenin[J]. Nature Communications, 2022.(IF 17.694)
8 Li Y, Zhao L, Zhang Y, et al. Structural basis for product specificities of MLL family methyltransferases[J]. Molecular Cell, 2022.(IF 19.328)
9 Lei Z, Meng H, Liu L, et al. Mitochondrial base editor induces substantial nuclear off-target mutations[J]. Nature, 2022.(IF 69.50)
10 Lin Q, Jin S, Zong Y, et al. High-efficiency prime editing with optimized, paired pegRNAs in plants[J]. Nature Biotechnology, 2021.(IF 54.90)
11 Song B, Chen Y, Liu X, et al. Ordered assembly of the cytosolic RNA-sensing MDA5-MAVS signaling complex via binding to unanchored K63-linked poly-ubiquitin chains[J]. Immunity, 2021.(IF 31.74)
12 Zong Y, Liu Y, Xue C, et al. An engineered prime editor with enhanced editing efficiency in plants[J]. Nature Biotechnology, 2022.(IF 54.00)
13 Li C, Zhang R, Meng X, et al. Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors[J]. Nature biotechnology, 2020.(IF 35.72)
14 Lin Q, Zong Y, Xue C, et al. Prime genome editing in rice and wheat[J]. Nature biotechnology, 2020.(IF 31.90)
15 Wang S, Zong Y, Lin Q, et al. Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9[J]. Nature biotechnology, 2020.(IF 36.55)
16 Chen J, Ou Y, Yang Y, et al. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing[J]. Nature, 2018.(IF 40.13)
