產(chǎn)品詳情介紹
CD4又稱T細胞表面抗原T4/Leu-3（T-cell surface antigen T4/Leu-3），是一個51 kDa的跨膜糖蛋白，分布于輔助性T細胞，巨噬細胞、樹突細胞或 NK細胞等細胞表面，在T細胞發(fā)育及激活過程中起到重要作用[1-4]。CD4是T細胞受體（T cell receptor,TCR）的共受體，與MHC Ⅱ類分子相結(jié)合，穩(wěn)定TCR與抗原肽-MHC復(fù)合物的結(jié)合。在輔助性T細胞中，招募淋巴細胞特異性蛋白酪氨酸激酶Lck至TCR-CD3復(fù)合物上，通過磷酸化多種底物起始不同的細胞內(nèi)信號通路，參與細胞激活、遷移、釋放細胞因子等[5]。在巨噬細胞或NK細胞，CD4通過非Lck依賴途徑促進細胞激活和分化，釋放細胞因子[2,4]。本產(chǎn)品為小鼠抗人CD4單克隆抗體，用于FC方法特異性檢測人CD4蛋白。
CD系列產(chǎn)品介紹視頻
[1] Isobe M, Huebner K, Maddon PJ, et al. The gene encoding the T-cell surface protein T4 is located on human chromosome 12 [J]. Proc Natl Acad Sci U S A. 1986, 83(12): 4399-402.
[2] Zhen A, Krutzik SR, Levin BR, et al. CD4 ligation on human blood monocytes triggers macrophage differentiation and enhances HIV infection [J]. J Virol. 2014, 88(17): 9934-46.
[3] Winkel K, Sotzik F, Vremec D, et al. CD4 and CD8 expression by human and mouse thymic dendritic cells [J]. Immunol Lett. 1994, 40(2): 93-9.
[4] Bernstein HB, Plasterer MC, Schiff SE, et al. CD4 Expression on Activated NK Cells: Ligation of CD4 Induces Cytokine Expression and Cell Migration [J]. The Journal of Immunology. 2006, 177(6): 3669-76.
[5] J?nsson P, Southcombe JH, Santos AM, et al. Remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions [J]. Proc Natl Acad Sci U S A. 2016, 113(20): 5682-7.
